ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of Tanyu Tongzhi (TYTZ) recipe on chemorheology, blood lipid and inflammatory factors of hyperlipidemia and myocardial ischemia-reperfusion injury in rats.</p><p><b>METHOD</b>Sixty SD male rats were divided into 5 groups randomly, sham-operated group, model group, high dose group of reproduced by ligation of left descending artery for 30 min followed by releasing the TYTZ and low group of TYTZ. The model of MI/RI injury of the myocardium was ligation for 2 hours in rats. Serum contents of CHO, TG, HDL-L, LDL-L and whole blood viscosity, plasma viscosity and ICAM-1, TNF-alpha, IL-10 were measured after myocardial reperfusion injury.</p><p><b>RESULT</b>Compared with sham-operated group, the levels of CHO, TG, LDL-L, whole blood viscosity (1.0,3.0) plasma viscosity and the contents of ICAM-1 were significantly higher, however, HDL-L, IL-10 levels were lower in model group (P < 0.01 and P < 0.05). CHO, TG, whole blood viscosity (1.0, 3.0, 30) and expression of ICAM-1, TNF-alpha were obviously lower in low group than the model group (P < 0.01 and P < 0.05).</p><p><b>CONCLUSION</b>The TYTZ recipe can relieve reperfusion injury through regulating blood lipid, improving hemorheological characteristic and inhibiting inflammatory reaction.</p>
Subject(s)
Animals , Male , Rats , Blood Proteins , Metabolism , Blood Viscosity , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Heart , Hemorheology , Hyperlipidemias , Drug Therapy , Inflammation , Blood , Lipids , Blood , Myocardial Reperfusion Injury , Drug Therapy , Random Allocation , Rats, Sprague-DawleyABSTRACT
Specificity of acupoints is one of the basic theories of the acupuncture and moxibustion sciences and is a very important basis to guide the clinical treatment of acupuncture and moxibustion. However, the scientific foundation of specificity of acupoints is still unclear, which has greatly restricted to the clinical effect improvement of acupuncture and moxibustion and has influenced to academic status of acupuncture and moxibustion sciences both in China and abroad. In this paper, the recent related researches are reviewed and then the key effects and mechanism that the tissue structure of acupoints can decide and affect specificity of acupoints-organs effects are analyzed and explored. It is held that acupuncture at the different tissue structure of the different depth in the different or the same acupoints will cause changes of varying qualities or degrees of visceral function because of stimulation of the different receptors in the different tissues followed by excitation of varying afferent nerve fibers.
Subject(s)
Animals , Humans , Acupuncture Points , Acupuncture Therapy , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To research on the protective effect of 3'-methoxy-puerarin (3'-mo-pue) on rat brain suffering from ischemia and to offer the experimental basis for widening the applied areas of Radix Puerariae.</p><p><b>METHOD</b>All rats were randomly divided into sham operation group, control group, Pue group and 3'-mo-pue group. The influence of different dosage on relevant indexes were measured through cerebral artery occlusion (MCAO) model rat covered by Fecl3, and cerebral ischemia-reperfusion model rat after 2VO.</p><p><b>RESULT</b>The result showed that 3'-mo-Pue could reduce the scores of neurological deficit, cerebral infarcted zone to varying degrees and the water content of brain tissue in MCAO model. It could obviously increase the activity of CAT and GPx in the hippocampus and also increase the activity of CAT and GPx in the cortex in the ischemia field. Moreover, 3'-mo-Pue could decrease the content of LPO, LD in the brain tissue of cerebral ischemia-reperfusion model rat.</p><p><b>CONCLUSION</b>3'-mo-Pue has favorable protective effect on brain suffering from ischemia. The mechanism is possibly related to its effect of anti-oxidation.</p>
Subject(s)
Animals , Male , Rats , Brain , Metabolism , Brain Ischemia , Drug Therapy , Metabolism , Catalase , Metabolism , Drugs, Chinese Herbal , Pharmacology , Enzyme Activation , Glutathione Peroxidase , Metabolism , Isoflavones , Pharmacology , Rats, Sprague-Dawley , Rats, WistarABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between renal tubular cells transdifferentiation and chronic renal interstitial fibrosis induced by Fangchi Extract in rat.</p><p><b>METHOD</b>The chronic renal interstitial fibrosis rat model was made by giving Radix Aristolochiae Fangchi extract (RAFE) and aristolichic acid (AA) respectively to rats through infusing stomach about 22 weeks discontinuously. Through immunnal histochemistry methods, investigating the expression of symbol proteins: Cytokine( CK) , alpha-Smooth muscle actin ( alpha-SMA) and Vimentin, and also the important fibrosis inducing factor-Transforming growth factor-beta (TGF-beta1 )on renal tubular cells.</p><p><b>RESULT</b>In RAFE and AA Groups, the expression of CK on renal tubular cells is declined comparing with the Control Group, and the enhanced expression of alpha-SMA and Vimentin can be observed on tubular cells. The expression of TGF-beta1 on renal tubular cells stronglhy increased, too.</p><p><b>CONCLUSION</b>Part of the renal tubular cells was transdifferentiated into myofibroblasts. Renal tubular cells may participate the occurance of chronic renal interstitial fibrosis, TGF-beta1 may accelerate the transdifferentiation of tubular cells.</p>
Subject(s)
Animals , Female , Male , Rats , Actins , Metabolism , Aristolochia , Chemistry , Aristolochic Acids , Toxicity , Cell Transdifferentiation , Cytokines , Metabolism , Drugs, Chinese Herbal , Toxicity , Epithelial Cells , Metabolism , Pathology , Fibrosis , Kidney Diseases , Metabolism , Kidney Tubules , Metabolism , Pathology , Plant Extracts , Therapeutic Uses , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Random Allocation , Rats, Sprague-Dawley , Transforming Growth Factor beta1 , Metabolism , Vimentin , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Qingkailing and Methylprednisolone (MP) injection alone or combined on the acute lung injury (ALI) induced by oleic acid in rabbits.</p><p><b>METHOD</b>The rabbits were randomly divided into 11 groups: oleic acid group; control group; treatment groups including low, middle and high dosage groups of Qingkailing and MP alone and combined, respectively. ALI model was established by i.v. oleic acid (0.05 mL x kg(-1)) in these groups, and then i.v. above drugs respectively, while in control group, the same volume of normal saline was given. The respiratory amplitude and rate were observed, and blood samples were taken from cervical artery for blood-gas analysis before and at 30, 60, 120 min after oleic acid or normal saline administration. At the end of experiment, the concentration of LDH, CAT and MDA in the lung tissue were measured and pathologic changes of lung tissue were observed microscopically.</p><p><b>RESULT</b>Compared with oleic acid group, the respiratory amplitude markedly enhanced (P < 0.05) and respiratory rate lowered (P < 0.05) in the low, middle and high dose groups of Qingkailing and MP injection. On the 30 min of treatment, PaO2 increased significantly (P < 0.05) in the low and middle dose groups of combined Qingkailing and MP injection; PaCO2 decreased markedly (P < 0.05) on the 120 min of treatment in each treatment group. The level of LDH significantly increased (P < 0.05), CAT and MDA decreased (P < 0.05) in the middle and high groups of Qingkailing and MP injection. The low and middle dose groups of combined Qingkailing and MP injection can alleviate the pathological changes induced by oleic acid.</p><p><b>CONCLUSION</b>The curative effect of the low dose group of combined Qingkailing and MP for the ALI induced by oleic acid was better than Qingkailing and MP alone, while the big dose groups of Qingkailing and MP alone better than the combination at the same dosage.</p>
Subject(s)
Animals , Female , Male , Rabbits , Anti-Inflammatory Agents , Therapeutic Uses , Blood Gas Analysis , Methods , Drug Therapy, Combination , Drugs, Chinese Herbal , Therapeutic Uses , Lung , Metabolism , Pathology , Methylprednisolone , Therapeutic Uses , Oleic Acid , Phytotherapy , Plants, Medicinal , Chemistry , Random Allocation , Respiratory Distress Syndrome , Drug Therapy , Metabolism , Respiratory Function TestsABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Qingikailing and Shengmai injection alone or combined on the acute lung injury (AL) induced by oleic acid in rabbits.</p><p><b>METHOD</b>The rabbits were randomly divided into 11 groups: oleic acid group; control group; treatment groups including low, middle and high dosage groups of Qingkailing and Shengmai injection alone and combined, respectively. ALI model was established by iv oleic acid (0.05 mL x kg(-1)) in these groups, and then iv above drugs respectively,while in control group, the same volume of normal saline was given. The respiratory amplitude and rate were observed, and blood samples were taken from cervical artery for blood-gas analysis before and at 30, 60, 120 min after oleic acid or normal saline administration. At the end of experiment, the concentration of LDH, CAT and MDA in the lung tissue were measured and pathologic changes of lung tissue were observed microscopically.</p><p><b>RESULT</b>Compared with oleic acid group, the respiratory amplitude markedly enhanced (P < 0.05) in the low and high dose groups of Qingkailing and Shengmai injection. PaO2 increased significantly (P < 0.05) in the low dose group of combined Qingkailing and Shengmai injection, PaCO2 decreased markedly (P < 0.05) in the low dose groups of Qingkailing and Shengmai injection alone and combined. The level of MDA significantly decreased (P < 0.05) in the each group of Qingkailing and Shengmai injection alone, the level of MDA significantly decreased (P < 0.05) and CAT increased (P < 0.05) in the low dose group of combined Qingkailing with Shengmai injection. The low dose group of combined Qingkailing and Shengmai injection can alleviate the pathological changes induced by oleic acid.</p><p><b>CONCLUSION</b>The curative effect of the low dose group of combined Qingkailing with Shengmai injection for the ALI induced by oleic acid was better than Qingkailing and Shengmai injection alone at the same dosage.</p>
Subject(s)
Animals , Female , Male , Rabbits , Carbon Dioxide , Blood , Catalase , Blood , Drug Combinations , Drug Therapy, Combination , Drugs, Chinese Herbal , Pharmacology , L-Lactate Dehydrogenase , Metabolism , Lung , Pathology , Malondialdehyde , Blood , Oleic Acid , Oxygen , Blood , Plants, Medicinal , Chemistry , Random Allocation , Respiration , Respiratory Distress Syndrome , Blood , PathologyABSTRACT
<p><b>OBJECTIVE</b>To discuss the protective effects of pueraria compound on the cerebral ischemic injury.</p><p><b>METHOD</b>Using the middle cerebral artery occlusion model (MCAO) in rats and cerebral ischemia-reperfusion models in gerbils and mice, we investigated the influence of pueraria compound on the brain water content and the infarct size, the cerebral apoplexy exponent, the contents of lactic acid (LA) and lipid peroxide (LPO), the activities of lactic dehydrogenase (LDH), glutathione peroxidase (GPx) and Na+ -K+ -ATPase.</p><p><b>RESULT</b>Pueraria compound obviously reduced the brain water content and the infrarct size in MCAO, improved motor abilities in the cerebral ischemia-reinfusion model of gerbils, decreased the contents of LA and LPO and increased the activities of LDH, GPx and Na+ -K+ -ATPase in cerebral ischemia-reinfusion model of mice.</p><p><b>CONCLUSION</b>Pueraria compound has the function of antioxidation and protective effect on ischemic brain tissue.</p>
Subject(s)
Animals , Male , Mice , Rats , Antioxidants , Pharmacology , Brain , Metabolism , Pathology , Brain Ischemia , Metabolism , Pathology , Drug Combinations , Gerbillinae , Glutathione Peroxidase , Metabolism , Isoflavones , Pharmacology , L-Lactate Dehydrogenase , Metabolism , Lactic Acid , Metabolism , Lipid Peroxides , Metabolism , Neuroprotective Agents , Pharmacology , Plants, Medicinal , Chemistry , Pueraria , Chemistry , Rats, Sprague-Dawley , Reperfusion Injury , Metabolism , Pathology , Sodium-Potassium-Exchanging ATPase , Metabolism , Soybeans , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the clinical effect of Tianzhi Granule (TZK) on senile vascular dementia (VaD), which is classified as sthenia of liver-yang.</p><p><b>METHOD</b>Two hundred VaD patients were treated with TZK (0.5 g/bag), which was taken one bag each, three times a day. The treatment course was one month and they were treated for rwo courses.</p><p><b>RESULT</b>TZK could remarkably increase gnosia and activity, with no striking difference from that of positive control group (P > 0.05). Simultaneously, TZK could significantly improve the clinical syndrome of traditional Chinese medicine and viability. It could also drastically reduce the whole blood and plasma viscosity and improve erythrodegeneration and abnormality of aggregation index in the abnormal blood viscosity patients.</p><p><b>CONCLUSION</b>TMC has certain effects on senile VaD.</p>